看見(jiàn)不少朋友在詢問(wèn)此類信息，這里簡(jiǎn)單說(shuō)一下，并提供一篇文章，供大家參考。 w,1*dn
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DIC即微分干涉相襯顯微鏡，其全稱是Differential and Interference Contrast . (
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其采用偏光原理，起偏鏡產(chǎn)生偏光后，就利用到諾馬斯基棱鏡了，即Nomarski，其作用是分解光束，分出兩束振動(dòng)方向一樣，等強(qiáng)度的垂直光束，相干面由Nomarski棱鏡的參數(shù)而定。觀察的圖像會(huì)極有立體感，觀察效果逼真。其特點(diǎn)是分辨率高，且能觀察透明物體。 XS> ( Bu
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Differential interference contrast microscopy (DIC), also known as Nomarski Interference Contrast (NIC) or Nomarski microscopy, is an optical microscopy illumination technique used to enhance the contrast in unstained, transparent samples. DIC works on the principle of interferometry to gain information about the optical density of the sample, to see otherwise invisible features. A relatively complex lighting scheme produces an image with the object appearing black to white on a grey background. This image is similar to that obtained by phase contrast microscopy but without the bright diffraction halo. <.
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DIC works by separating a polarised light source into two beams which take slightly different paths through the sample. Where the length of each optical path (i.e. the product of refractive index and geometric path length) differs, the beams interfere when they are recombined. This gives the appearance of a three-dimensional physical relief corresponding to the variation of optical density of the sample, emphasising lines and edges though not providing a topographically accurate image.
